Difference between revisions of "Part:BBa K143062"

Revision as of 02:45, 30 October 2008

LacI repressor protein - Terminator

LacI transcriptional repressor protein (BBa_K143033) coupled to the double terminator (BBa_B0015).

The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with B. subtilis. LacI can be used in conjunction with the lac operon promoter (BBa_K143015), where the LacI will act as a receiver for an IPTG input to result in a Polymerases per second (PoPS) output.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.

Note. This part has picked up a mutation, which changes an amino acid residue from a Serine to a Leucine but does not cause a frame shift. Given the size of the protein, this should not adversley afect its function to a significant degree.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 15:00, 1 October 2008 (view source) Chris (Talk \| contribs) m ← Older edit		Revision as of 02:45, 30 October 2008 (view source) Chris (Talk \| contribs) m Newer edit →
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	The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.		The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.
		+
		+	Note. This part has picked up a mutation, which changes an amino acid residue from a Serine to a Leucine but does not cause a frame shift. Given the size of the protein, this should not adversley afect its function to a significant degree.