Difference between revisions of "Part:BBa K3424022:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
a
+
When designing the system, make sure there are 4 nucleotides in between the terminator(BBa_K3424009) and the following promoter like pPhoA. The source link to this terminator also offers different sequences for assembling genetic circuits, if interested in using as well.
 +
 
  
  
Line 13: Line 14:
 
===Source===
 
===Source===
  
−
a
+
https://static-content.springer.com/esm/art%3A10.1038%2Fs41589-018-0168-3/MediaObjects/41589_2018_168_MOESM1_ESM.pdf?fbclid=IwAR22C9bYtULbPECeq8TFaAhzM7D4_RDMQ9SoeSoqnM6OdHqtg_-hpK6GoXE
 +
 
  
 
===References===
 
===References===
 +
A dynamic model of the phosphate response system with synthetic promoters in Escherichia coli Cansu Uluşeker , Jesus Torres , José L. García , Martin M. Hanczyc , Juan Nogales , and Ozan Kahramanoğulları Artificial Life Conference Proceedings 2017 NO. 29, 412-419
 +
 +
Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3
 +
Uluşeker C, Torres-Bacete J, García JL, Hanczyc MM, Nogales J, Kahramanoğulları O. Quantifying dynamic mechanisms of auto-regulation in Escherichia coli with synthetic promoter in response to varying external phosphate levels. Sci Rep. 2019 Feb 14;9(1):2076. doi: 10.1038/s41598-018-38223-w. PMID: 30765722; PMCID: PMC6376016.

Revision as of 19:14, 27 October 2020


PhoBR Circuit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2984
    Illegal SapI.rc site found at 535


Design Notes

When designing the system, make sure there are 4 nucleotides in between the terminator(BBa_K3424009) and the following promoter like pPhoA. The source link to this terminator also offers different sequences for assembling genetic circuits, if interested in using as well.



Source

https://static-content.springer.com/esm/art%3A10.1038%2Fs41589-018-0168-3/MediaObjects/41589_2018_168_MOESM1_ESM.pdf?fbclid=IwAR22C9bYtULbPECeq8TFaAhzM7D4_RDMQ9SoeSoqnM6OdHqtg_-hpK6GoXE


References

A dynamic model of the phosphate response system with synthetic promoters in Escherichia coli Cansu Uluşeker , Jesus Torres , José L. García , Martin M. Hanczyc , Juan Nogales , and Ozan Kahramanoğulları Artificial Life Conference Proceedings 2017 NO. 29, 412-419

Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3 Uluşeker C, Torres-Bacete J, García JL, Hanczyc MM, Nogales J, Kahramanoğulları O. Quantifying dynamic mechanisms of auto-regulation in Escherichia coli with synthetic promoter in response to varying external phosphate levels. Sci Rep. 2019 Feb 14;9(1):2076. doi: 10.1038/s41598-018-38223-w. PMID: 30765722; PMCID: PMC6376016.