Difference between revisions of "Part:BBa K3505023"

Revision as of 19:12, 27 October 2020

FFAR2:V2tail:TCS GB compatible with B2-B3

Usage and Biology

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B2-B5.

Figure 1.The overhangs of this part in the Golden Braid Grammar.

Figure 2. The level 0 module : pupd2-FFAR2:V2tail:TCS (illustration from SnapGene)

Verification of Cloning

Fig.1:(U=Uncut C=Cut) Restriction Digestion of FFAR2:V2tail:TCS with BamHI, Expected bands : 3204 bp, Positive Result:C2,C3

Source

Synthesized by IDT.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 284
Illegal PstI site found at 864
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 284
Illegal PstI site found at 864
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 106
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 284
Illegal PstI site found at 864
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 284
Illegal PstI site found at 864
Illegal NgoMIV site found at 364
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 560
Illegal SapI site found at 815
Illegal SapI.rc site found at 17

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3505023 short</partinfo>
-long
@@ Line 22: / Line 22: @@
 ===Verification of Cloning===
 [[File:T--Thessaly--FFAR2-ECFP-digestion.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction Digestion of FFAR2:V2tail:TCS with BamHI, Expected bands : 3204 bp, Positive Result:C2,C3</i>]]
+===Source===
+Synthesized by IDT.
 ===Sequence and Features===
 <partinfo>BBa_K3505023 SequenceAndFeatures</partinfo>