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Revision as of 19:07, 27 October 2020


pTrc-2-RBS-ECFP-terminator

With this part, the pTrc-2 promoter can be tested by observing the fluorescence intensity/OD.

Usage and Biology

This part can be used to test that if the pTrc-2 promoter can work. The fluorescence intensity can reflect the strength of the pTrc-2 promoter.

Fig 1. pTrc-2_RBS(B0034)_ECFP(E0020)_terminator(B0015)

Characterization

The agarose gel electrophoresis images are below:

caption

Fig 2. pTrc-2_E0420_pSB1C3 digested by Xba I and Pst I(about 1003bp)

Reference

[1] Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ. 'Deadman' and 'Passcode' microbial kill switches for bacterial containment. Nat Chem Biol. 2016;12(2):82-86. doi:10.1038/nchembio.1979


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]