Difference between revisions of "Part:BBa K3505017"
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[[File:T--Thessaly--terminator-teliko-digestion.png|700px|thumb|none|<i><b>Fig.3:</b>(U=Uncut C=Cut) Restriction digestion of Terminator (C3 )with :  
 
[[File:T--Thessaly--terminator-teliko-digestion.png|700px|thumb|none|<i><b>Fig.3:</b>(U=Uncut C=Cut) Restriction digestion of Terminator (C3 )with :  
 
EcoRI & PstI ( C3 ), Expected bands : 2029 + 209 bp , Positive result : C3</i>]]
 
EcoRI & PstI ( C3 ), Expected bands : 2029 + 209 bp , Positive result : C3</i>]]
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===Source===
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Synthesized by IDT.

Revision as of 18:59, 27 October 2020


Terminator.png

Double Terminator GB compatible with B6-C1

biologyrrnBT1-T7TE
chassisE.coli
directionForward
forward_efficiency0.984[CC]
0.97[JK]
reversed_version2146
reverse_efficiency0.295[CC]
0.62[JK]

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B6-C1.

Figure 1.The overhangs of this part in the Golden Braid Grammar.
Figure 2. The level 0 module : pupd2- double terminator. (illustration from SnapGene)

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction digestion of Terminator (C3 )with : EcoRI & PstI ( C3 ), Expected bands : 2029 + 209 bp , Positive result : C3

Source

Synthesized by IDT.