Difference between revisions of "Part:BBa K3621000:Design"

 
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===Design Notes===
 
===Design Notes===
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Used mammalian MoClo system
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This part was planned and designed online for use in our computational model. See more on the <html> <a href="https://2020.igem.org/Team:MIT/Model">Model Page on the MIT iGEM 2020 wiki. </a>
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</html>
  
  

Revision as of 18:40, 27 October 2020


CasE-> cleaves cut site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 160
    Illegal PstI site found at 232
    Illegal PstI site found at 376
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 160
    Illegal PstI site found at 232
    Illegal PstI site found at 376
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 160
    Illegal PstI site found at 232
    Illegal PstI site found at 376
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 160
    Illegal PstI site found at 232
    Illegal PstI site found at 376
    Illegal NgoMIV site found at 516
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was planned and designed online for use in our computational model. See more on the Model Page on the MIT iGEM 2020 wiki.


Source

Sequence

References