Difference between revisions of "Part:BBa K3505016"
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===Design Notes=== | ===Design Notes=== | ||
| − | The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 < | + | The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 <bbpart>BBa_K3505007 </bpart> and has overhangs compatible for Golden Braid cloning. |
The CDS has position A1-B2 | The CDS has position A1-B2 | ||
Revision as of 18:40, 27 October 2020
araC-ParaBAD:RBS GB compatible with A1-B1
long
Usage and Biology
The PBAD promoter, derived from the arabinose operon andis an inducible promoter for arabinose. The use of an inducible promoter provides a most reversible and flexible gene circuit and at the same time exhibits a higher efficiency and lower side effects as cell death. The araC gene, is a regulatory gene and is located upstream of the L-arabinose operon, encodes a positive regulatory protein AraC, required for L-arabinose utilization in Escherichia coli. The araC gene has a constitutive promoter.
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 BBa_K3505007