Difference between revisions of "Part:BBa K3621014"
 
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What does it do?: It's responsible for the expression of the endoribonuclease, Cas6 in the presence of ELK-1. It was important for our project in particular because of its involvement in the PERSIST regulation mechanism.
 
What does it do?: It's responsible for the expression of the endoribonuclease, Cas6 in the presence of ELK-1. It was important for our project in particular because of its involvement in the PERSIST regulation mechanism.
  
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How to use it?: This part was used in our Immunomodulation System and computational model. See more on the
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How to use it?: This part was used in our immunomodulation system and computational model. See more on the
 
<html> <a href="https://2020.igem.org/Team:MIT">MIT iGEM 2020 wiki</a>
 
<html> <a href="https://2020.igem.org/Team:MIT">MIT iGEM 2020 wiki</a>
 
</html>
 
</html>

Latest revision as of 18:35, 27 October 2020


ELK-1 Responsive Promoter, CasE

Contents: ELK-1 Responsive Promoter, Cas6, SynthpA

What is it?: A Composite part; it is a genetic circuit with ELK-1 responsive promoter and Cas6 gene allowing for Cas6 expression when ELK-1 is present

What does it do?: It's responsible for the expression of the endoribonuclease, Cas6 in the presence of ELK-1. It was important for our project in particular because of its involvement in the PERSIST regulation mechanism.

How to use it?: This part was used in our immunomodulation system and computational model. See more on the MIT iGEM 2020 wiki


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1299
    Illegal PstI site found at 850
    Illegal PstI site found at 922
    Illegal PstI site found at 1066
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 850
    Illegal PstI site found at 922
    Illegal PstI site found at 1066
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1299
    Illegal PstI site found at 850
    Illegal PstI site found at 922
    Illegal PstI site found at 1066
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1299
    Illegal PstI site found at 850
    Illegal PstI site found at 922
    Illegal PstI site found at 1066
    Illegal NgoMIV site found at 1206
    Illegal AgeI site found at 1305
  • 1000
    COMPATIBLE WITH RFC[1000]