Difference between revisions of "Part:BBa K3621015"

Revision as of 18:31, 27 October 2020

NF-kB Responsive Promoter, Csy4

Contents: NF-kB Responsive Promoter, Csy4, SynthpA

What is it?: A Composite part; it is a genetic circuit with NF-kB responsive promoter and Csy4 gene allowing for Csy4 expression when NF-kB is present

What does it do?: It's responsible for the expression of the endoribonuclease, Csy4 in the presence of NF-kB. It was important for our project in particular because of its involvement in the PERSIST regulation mechanism.

How to use it?: This part was used in our Immunomodulation System and computational model. See more on the MIT iGEM 2020 wiki: https://2020.igem.org/Team:MIT

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1284
Illegal PstI site found at 1201
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1201
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 753
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1284
Illegal PstI site found at 1201
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1284
Illegal PstI site found at 1201
Illegal AgeI site found at 1290
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 What does it do?: It's responsible for the expression of the endoribonuclease, Csy4 in the presence of NF-kB. It was important for our project in particular because of its involvement in the PERSIST regulation mechanism.
-How to use it?: See more on the Parts Overview Page or on the MIT iGEM 2020 wiki: https://2020.igem.org/Team:MIT.
+How to use it?: This part was used in our Immunomodulation System and computational model. See more on the MIT iGEM 2020 wiki: https://2020.igem.org/Team:MIT
 <!-- Add more about the biology of this part here