Difference between revisions of "Part:BBa K3621015"
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What does it do?: It's responsible for the expression of the endoribonuclease, Csy4 in the presence of NF-kB. It was important for our project in particular because of its involvement in the PERSIST regulation mechanism. | What does it do?: It's responsible for the expression of the endoribonuclease, Csy4 in the presence of NF-kB. It was important for our project in particular because of its involvement in the PERSIST regulation mechanism. | ||
| − | How to use it?: See more on the Parts Overview Page or on the <a href=https://2020.igem.org/Team:MIT> MIT iGEM 2020 wiki </a> | + | How to use it?: See more on the Parts Overview Page or on the <a href="https://2020.igem.org/Team:MIT"> MIT iGEM 2020 wiki </a> |
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Revision as of 18:29, 27 October 2020
NF-kB Responsive Promoter, Csy4
Contents: NF-kB Responsive Promoter, Csy4, SynthpA
What is it?: A Composite part; it is a genetic circuit with NF-kB responsive promoter and Csy4 gene allowing for Csy4 expression when NF-kB is present
What does it do?: It's responsible for the expression of the endoribonuclease, Csy4 in the presence of NF-kB. It was important for our project in particular because of its involvement in the PERSIST regulation mechanism.
How to use it?: See more on the Parts Overview Page or on the <a href="https://2020.igem.org/Team:MIT"> MIT iGEM 2020 wiki </a>
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1284
Illegal PstI site found at 1201 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1201
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 753
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1284
Illegal PstI site found at 1201 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1284
Illegal PstI site found at 1201
Illegal AgeI site found at 1290 - 1000COMPATIBLE WITH RFC[1000]