Difference between revisions of "Part:BBa K3482008"

Latest revision as of 18:23, 27 October 2020

Non-structural maintenance of chromosomes element 1 (Nse1)

Usage and Biology

Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. This part was used as a control for the 3xFLAG tag in western blot experiments.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 37
Illegal PstI site found at 172
Illegal PstI site found at 388
Illegal PstI site found at 430
Illegal PstI site found at 619
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 37
Illegal PstI site found at 172
Illegal PstI site found at 388
Illegal PstI site found at 430
Illegal PstI site found at 619
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 548
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 37
Illegal PstI site found at 172
Illegal PstI site found at 388
Illegal PstI site found at 430
Illegal PstI site found at 619
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 37
Illegal PstI site found at 172
Illegal PstI site found at 388
Illegal PstI site found at 430
Illegal PstI site found at 619
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.
+Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. This part was used as a control for the 3xFLAG tag in western blot experiments.
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