Difference between revisions of "Part:BBa K3450016:Design"
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Removed the extra bases upstream to the start codon, stop codon, and extra bases downstream to the stop codon. | Removed the extra bases upstream to the start codon, stop codon, and extra bases downstream to the stop codon. | ||
| + | Codon optimized for Escherichia coli. | ||
| + | Removed the BsaI site from the sequence to make it RFC1000 compatible. | ||
===Source=== | ===Source=== | ||
Latest revision as of 18:21, 27 October 2020
Coding sequence of TEV Protease with a single S219V mutation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Removed the extra bases upstream to the start codon, stop codon, and extra bases downstream to the stop codon.
Codon optimized for Escherichia coli.
Removed the BsaI site from the sequence to make it RFC1000 compatible.
Source
TEV virus genome