Difference between revisions of "Part:BBa K3424023:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
a
+
 
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
a
+
Part:BBa_K3086001
 +
 
 +
https://static-content.springer.com/esm/art%3A10.1038%2Fs41589-018-0168-3/MediaObjects/41589_2018_168_MOESM1_ESM.pdf?fbclid=IwAR22C9bYtULbPECeq8TFaAhzM7D4_RDMQ9SoeSoqnM6OdHqtg_-hpK6GoXE
 +
 
  
 
===References===
 
===References===
 +
 +
Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3

Latest revision as of 17:43, 27 October 2020


pTet-SCRIBE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1879
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1284
    Illegal XhoI site found at 518
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1879
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Part:BBa_K3086001

https://static-content.springer.com/esm/art%3A10.1038%2Fs41589-018-0168-3/MediaObjects/41589_2018_168_MOESM1_ESM.pdf?fbclid=IwAR22C9bYtULbPECeq8TFaAhzM7D4_RDMQ9SoeSoqnM6OdHqtg_-hpK6GoXE


References

Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3