Difference between revisions of "Part:BBa K3482005"
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| − | The signal peptide 4 (NSP4) was selected because it should improve the secretion of a targeted protein. This peptide is part of the Sec-dependent secretion pathway which is the main secretion pathway in E.coli. This NSP4 peptide fused to the N-terminus of a recombinant protein promotes its translocation across the plasma membrane. This peptide was choosen by our team to enhance azurin export in our E.coli Nissle 1917 strain. | + | The signal peptide 4 (NSP4) was selected because it should improve the secretion of a targeted protein. This peptide is part of the Sec-dependent secretion pathway which is the main secretion pathway in <i>E.coli. This NSP4 peptide fused to the N-terminus of a recombinant protein promotes its translocation across the plasma membrane. This peptide was choosen by our team to enhance azurin export in our <i>E.coli Nissle 1917 strain. |
Revision as of 17:37, 27 October 2020
NSP4 secretion signal peptide
This part codes for a signal peptide, which can be fused to the N-terminus of a polypeptide in order to export the newly synthesised protein out of the cell.
Usage and Biology
The signal peptide 4 (NSP4) was selected because it should improve the secretion of a targeted protein. This peptide is part of the Sec-dependent secretion pathway which is the main secretion pathway in E.coli. This NSP4 peptide fused to the N-terminus of a recombinant protein promotes its translocation across the plasma membrane. This peptide was choosen by our team to enhance azurin export in our <i>E.coli Nissle 1917 strain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 48
- 1000COMPATIBLE WITH RFC[1000]