Difference between revisions of "Part:BBa K3605003"

 
 
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<partinfo>BBa_K3605003 short</partinfo>
  
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This part is bacterial cellulose synthase (Bcs) complex (9102bp) from Gluconacetobacter xylinus. It contains 4 subunits BcsA, BcsB, BcsC and BcsD, which catalyzes the bacterial cellulose synthesis, using uridine-5’-phosphate-α-D-glucose (UDPG). This is the last reaction for Bacterial cellulose production from glucose. The reaction catalyzed by this enzyme is as follows:
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[[File:K3605003-1.jpg|center]]
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Due to the long sequence (9102bp), this enzyme complex was synthesized directly and connected into pUC57 vector firstly, then it was inserted into the plasmid pSB1C3. The identification result is showed in Fig.1.
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[[File:K3605003-2.jpg|center]]
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Fig.1. The gene cloning result of Bcs complex.
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M: Marker; 1: Digestion of pUC57 vector containing Bcs complex; 2: Digestion of pSB1C3 vector containing Bsc complex.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3605003 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3605003 parameters</partinfo>
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Latest revision as of 17:29, 27 October 2020

Bacteria cellulose synthase (Bcs) from Gluconacetobacter xylinus.

This part is bacterial cellulose synthase (Bcs) complex (9102bp) from Gluconacetobacter xylinus. It contains 4 subunits BcsA, BcsB, BcsC and BcsD, which catalyzes the bacterial cellulose synthesis, using uridine-5’-phosphate-α-D-glucose (UDPG). This is the last reaction for Bacterial cellulose production from glucose. The reaction catalyzed by this enzyme is as follows:

K3605003-1.jpg



Due to the long sequence (9102bp), this enzyme complex was synthesized directly and connected into pUC57 vector firstly, then it was inserted into the plasmid pSB1C3. The identification result is showed in Fig.1.

K3605003-2.jpg

Fig.1. The gene cloning result of Bcs complex. M: Marker; 1: Digestion of pUC57 vector containing Bcs complex; 2: Digestion of pSB1C3 vector containing Bsc complex.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 740
    Illegal BglII site found at 1136
    Illegal XhoI site found at 232
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3387
    Illegal NgoMIV site found at 4617
    Illegal NgoMIV site found at 4687
    Illegal NgoMIV site found at 5569
    Illegal NgoMIV site found at 5758
    Illegal NgoMIV site found at 8925
    Illegal NgoMIV site found at 8929
    Illegal AgeI site found at 7281
    Illegal AgeI site found at 7558
    Illegal AgeI site found at 7818
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6141
    Illegal BsaI.rc site found at 982
    Illegal BsaI.rc site found at 1705