Difference between revisions of "Part:BBa K133008"

Revision as of 01:38, 30 October 2008

CF213-UB33

This part is composed of CF213 – the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)

UB33 is a peptide derived from the catalytic site of beta subunit of H. pylori urease. Urease is an important pathogenicity factor for H. pylori since it enables the bacteria to breakdown of urea into ammonia and carbon dioxide, thus artificially buffering the acidic enviroment in the stomach.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 718
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 301
1000
COMPATIBLE WITH RFC[1000]

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 CF213 – the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal  portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)
-We also designed synthetic multiepitope, comprising the H. pylori urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Multiepitope is a synthetic gene orderd from GeneArt.
+UB33 is a peptide derived from the catalytic site of beta subunit of H. pylori urease. Urease is an important pathogenicity factor for H. pylori since it enables the bacteria to breakdown of urea into ammonia and carbon dioxide, thus artificially buffering the acidic enviroment in the stomach.
 <!-- Add more about the biology of this part here