Difference between revisions of "Part:BBa K3605002"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K3605002 short</partinfo> | ||
+ | This part contains two enzymes (2277bp), which is a polycistron in original organism. One of them is phosphoglucomutase (PGM), it can isomerize the glucose-6-phosphate (G-6-P) to glucose-1-phosphate (G-1-P), which is the second reaction for bacterial cellulose production from glucose. Another enzyme is UDP-glucose pyrophosphorylase (UGPase), it can catalyze the glucose-1-phosphate (G-1-P) to react with UTP, forming uridine-5’-phosphate-α-D-glucose (UDPG), which is the third reaction for Bacterial cellulose production from glucose. | ||
+ | <br/> | ||
+ | [[File:K3605002-1.jpg|center]] | ||
+ | <br/> | ||
+ | <br/> | ||
+ | These two enzymes were also amplified using PCR method, and inserted into the vector pSB1C3. Fig.1 shows the identification result. | ||
+ | <br/> | ||
+ | [[File:K3605002-2.jpg|center]] | ||
+ | Fig.1. The result of PGM and UGPase gene cloning. | ||
+ | M: Marker; 1: PCR result of PGM and UGPase; 2: Digestion of pSB1C3 containing PGM and UGPase genes. | ||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K3605001 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K3605001 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 17:09, 27 October 2020
Phosphoglucomutase (PGM) and UDP-glucose pyrophosphorylase (UGPase) from Gluconacetobacter xylinus.
This part contains two enzymes (2277bp), which is a polycistron in original organism. One of them is phosphoglucomutase (PGM), it can isomerize the glucose-6-phosphate (G-6-P) to glucose-1-phosphate (G-1-P), which is the second reaction for bacterial cellulose production from glucose. Another enzyme is UDP-glucose pyrophosphorylase (UGPase), it can catalyze the glucose-1-phosphate (G-1-P) to react with UTP, forming uridine-5’-phosphate-α-D-glucose (UDPG), which is the third reaction for Bacterial cellulose production from glucose.
These two enzymes were also amplified using PCR method, and inserted into the vector pSB1C3. Fig.1 shows the identification result.
Fig.1. The result of PGM and UGPase gene cloning. M: Marker; 1: PCR result of PGM and UGPase; 2: Digestion of pSB1C3 containing PGM and UGPase genes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 548
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 478