Difference between revisions of "Part:BBa K3406017"
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− | The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas13 protein, and different Cas13 proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to PsmCas13b and SARS- | + | The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas13 protein, and different Cas13 proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to PsmCas13b and SARS-CoV-2, which could combine with PsmCas13b protein and guide it to target SARS-CoV-2 sequence, thus activating the collateral cleavage of PsmCas13b protein. |
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Latest revision as of 16:53, 27 October 2020
PsmCas13b-SARS-CoV-2-crRNA
The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas13 protein, and different Cas13 proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to PsmCas13b and SARS-CoV-2, which could combine with PsmCas13b protein and guide it to target SARS-CoV-2 sequence, thus activating the collateral cleavage of PsmCas13b protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]