Difference between revisions of "Part:BBa K3406015"
| Line 3: | Line 3: | ||
<partinfo>BBa_K3406015 short</partinfo> | <partinfo>BBa_K3406015 short</partinfo> | ||
| − | The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas protein, and different Cas proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to LwaCas13a and SARS- | + | The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas protein, and different Cas proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to LwaCas13a and SARS-CoV, which could combine with LwaCas13a protein and guide it to target SARS-CoV sequence, thus activating the collateral cleavage of LwaCas13a protein. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 16:50, 27 October 2020
LwaCas13a-SARS-CoV-crRNA
The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas protein, and different Cas proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to LwaCas13a and SARS-CoV, which could combine with LwaCas13a protein and guide it to target SARS-CoV sequence, thus activating the collateral cleavage of LwaCas13a protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]