Difference between revisions of "Part:BBa K3505033"

Revision as of 16:45, 27 October 2020

pAndersonJ23115:RBS:tetR-terminator

Usage and Biology

This parts consists of AndersonJ23115 promoter BBa_K3505012 , TetR BBa_K3505005 and Double Terminator BBa_K3505017. By this way ,as AndersonJ23115 is a strong constitutive promoter, TetR is constitutively expressed. TetR can bind to tetracycline response element and inhibits the expression of a desirable transcription unit

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector BBa_K3505008 and has overhangs compatible for Golden Braid cloning.

Figure 2. The level A module of Tet-Off system : AndersonJ23115:RBS-TetR-Double terminator

Verification of Cloning

Fig.1:(U=Uncut C=Cut) Restriction digestion of T: AndersonJ23115-TetR-double terminator(C3-C4 ) with: HindIII (C3-C4) , Expected bands : 2847 + 836 bp ,Positive result : C3,C4

Sequence and Features