Difference between revisions of "Part:BBa K3332013"

Revision as of 16:33, 27 October 2020

Lpp-OmpA-GRHPR

        We anchor GRHPR protein onto membranes through Lpp-OmpA to catalyze the reaction of reducing glyoxalic acid and consuming NADPH. We use BBa_K880005 to construct the expression system and anchor GRHPR on the surface of E.coli.

Biology

        Lpp-OmpA is an anchor protein from E. coli, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. GRHPR, a Glyoxylate reductase from human liver, can reduce glyoxylic acid when NADPH is used as cofactor. GRHPR is fused at N terminal with Lpp-OmpA so that GRHPR can be displayed on the surface of E. coli.^[1][2]

Fig 1. mechanism.

Usage

        Here, we used BBa_K880005 to construct the expression system and demonstrate the effect of Lpp-OmpA-GRHPR on the surface of E. coli. We obtained the composite part BBa_K3332058. Due to the limited time, we didn’t get the gene in time. As a result, there is a lack of data about this part. The progress of this part remains in the stage of design.

Fig 2. Gene circuit of Lpp-OmpA-GRHPR.

References

1. ↑ Rumsby G, Cregeen D P. Identification and expression of a cDNA for human hydroxypyruvate/glyoxylate reductase[J]. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1999, 1446(3): 383-388.
2. ↑ http://2016.igem.org/Team:TJUSLS_China

Sequence and Features