Difference between revisions of "Part:BBa K3402050"

Revision as of 16:14, 27 October 2020

report circuit

This device is composed of promoter Ptef1 (BBa_K3402007), reporter gene yeGFP (BBa_K3402000), terminator Tsyn7 (BBa_K3402001).

Usage and Biology

The green fluorescent protein (yeGFP) gene can be used as reporter gene and express in Starmerella bombocola. We conducted this device to test if the yeGFP can express in Starmerella bombocola, so we connected this device. Besides, the hygromycin resistance gene (hph) was built into the plasmid as the marker gene to determine if the transformation is successful. Two homologous arms are used to intsert this gene into PXA1 site.
As a result, the yeGFP was succefully expressed in Starmerella bombocola. Finally, we can link different promoters to yeGFP to know the strength of promoters by testing different fluorescence intensity and transcription levels analysis of different strains.

a) Fluorescence intensity of different promoters. b) Transcription levels of different promoters. c) GFP was successfully expressed in cells

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1051
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1492

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 ===Usage and Biology===
-The green fluorescent protein (<i>yeGFP</i>) gene can be used as reporter gene and express in <i>Starmerella bombocola</i>. We want to test if the <i>yeGFP</i> can express in <i>Starmerella bombocola</i>, so we connected this device. Besides, the hygromycin resistance gene (<i>hph</i>) was built into the plasmid as the marker gene to determine if the transformation is successful. Two homologous arms are used to intsert this gene into PXA1 gene.
+The green fluorescent protein (<i>yeGFP</i>) gene can be used as reporter gene and express in <i>Starmerella bombocola</i>. We conducted this device to test if the <i>yeGFP</i> can express in <i>Starmerella bombocola</i>, so we connected this device. Besides, the hygromycin resistance gene (<i>hph</i>) was built into the plasmid as the marker gene to determine if the transformation is successful. Two homologous arms are used to intsert this gene into <i>PXA1</i> site.
 <br>
-As a result, the expression of the <i>yeGFP</i> in <i>Starmerella bombocola</i> is completed. Finally, we can link different promoters to <i>yeGFP</i> to know the strength of promoters by testing different fluorescence intensity of different promoters.
+As a result, the <i>yeGFP</i> was succefully expressed in <i>Starmerella bombocola</i>. Finally, we can link different promoters to <i>yeGFP</i> to know the strength of promoters by testing different fluorescence intensity and transcription levels analysis of different strains.
 [[Image:Expression of yeGFP abc.png|700px|thumb|center|a) Fluorescence intensity of different promoters.  b) Transcription levels of different promoters.  c) GFP was successfully expressed in cells]]