Difference between revisions of "Part:BBa K3365017:Design"
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===Source=== | ===Source=== | ||
| − | + | The ligation of BBa_K3365003, BBa_K3365054 and BBa_K3365002 by Overlap PCR. | |
===References=== | ===References=== | ||
Revision as of 15:43, 27 October 2020
pBAD upstream of eGFP with inhibition unit containing lure3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1053
Illegal XhoI site found at 1062 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 74
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 56
Design Notes
The restriction cutting sites are added to the fragment during PCR for easy ligation to the backbone.
Source
The ligation of BBa_K3365003, BBa_K3365054 and BBa_K3365002 by Overlap PCR.