Difference between revisions of "Part:BBa J31005"
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| + | ===Chloramphenicol resistance=== | ||
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| + | Chloramphenicol concentrations up to 75μg/ml can be tolerated by bacteria carrying a single copy of the Chloramphenicol acetyltransferase gene, though ideally cells would be grown in medium with a chloramphenicol concentration of less than 30μg/ml. | ||
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| + | [[Image:CAT_Working_Conditions.PNG|center]] | ||
Revision as of 01:16, 30 October 2008
chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004]
When a promoter and an RBS are in front of the gene, the cell will express Chloramphenicol resistance. Because it contains full biobrick ends, this part can be used to easily add chloramphenicol resistance to any part without changing plasmid vectors.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Chloramphenicol resistance
Chloramphenicol concentrations up to 75μg/ml can be tolerated by bacteria carrying a single copy of the Chloramphenicol acetyltransferase gene, though ideally cells would be grown in medium with a chloramphenicol concentration of less than 30μg/ml.
