Difference between revisions of "Part:BBa K133002"
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Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of ''E. coli'' with that of ''H. pylori'' enables activation of innate immunity - a characteristic that flagellin of ''H. pylori'' alone does not posess - while presenting the antigen of ''H. pylori''  for antibody production.
 
Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of ''E. coli'' with that of ''H. pylori'' enables activation of innate immunity - a characteristic that flagellin of ''H. pylori'' alone does not posess - while presenting the antigen of ''H. pylori''  for antibody production.
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Since the protein was designed to be produced, we additionally added RGD-Hisstop tag (for the part description and implication follow: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K133036].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:05, 30 October 2008

CF-RGD_Histop

Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production.

Since the protein was designed to be produced, we additionally added RGD-Hisstop tag (for the part description and implication follow: [1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1207
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 764
    Illegal BamHI site found at 1275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1558