Difference between revisions of "Part:BBa K3380630:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This construct has not been tested in the lab, however the Edinburgh iGEM 2020 team tested the BBa_K3380500 construct. The BBa_K3380630 construct can be assembled following a similar protocol (on the experience page of the BBa_K3380500 construct). | |
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| + | All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase. | ||
===Source=== | ===Source=== | ||
Latest revision as of 15:01, 27 October 2020
TXO Fluoride riboswitch regulated iSpinach construct under Anderson Promoter (BBa_J23119)
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This construct has not been tested in the lab, however the Edinburgh iGEM 2020 team tested the BBa_K3380500 construct. The BBa_K3380630 construct can be assembled following a similar protocol (on the experience page of the BBa_K3380500 construct).
All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase.
Source
-
References
Thavarajah, W., Silverman, A.D., Verosloff, M.S., Kelley-Loughnane, N., Jewett, M.C. and Lucks, J.B., 2019. Point-of-use detection of environmental fluoride via a cell-free riboswitch-based biosensor. ACS synthetic biology, 9(1), pp.10-18.