Difference between revisions of "Part:BBa K3424001:Design"
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=== Plasmid Design Notes===
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__NOTOC__
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<partinfo>BBBa_K3424001 short</partinfo>
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<partinfo>BBa_K3424001 SequenceAndFeatures</partinfo>
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===Design Notes===
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N/A
  
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Make sure to check the source paper and type in the sequence of the terminator to see how the authors designed it. If you want to design another gene after this terminator in your plasmid, you should have around 19 intermediate bases before inserting a promoter for your other gene.
 
  
  
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Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3
 
Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3
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===References===

Revision as of 14:42, 27 October 2020

No part name specified with partinfo tag.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 46


Design Notes

N/A


Source

Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3


References