Difference between revisions of "Part:BBa K3599035"
 
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===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K3599035 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3599035 SequenceAndFeatures</partinfo>
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===Reference===
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Kang, S. Y., Choi, O., Lee, J. K., Ahn, J. O., Ahn, J. S., Hwang, B. Y., & Hong, Y. S. (2015). Artificial de novo biosynthesis of hydroxystyrene derivatives in a tyrosine overproducing Escherichia coli strain. <i>Microbial cell factories</i>, 14(1), 1-11. [https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-015-0268-7 10.1186/s12934-015-0268-7]

Latest revision as of 14:41, 27 October 2020


baPAD

an enzyme which was designed into the artificial biosynthesis pathway of 4VA synthesis. The original function of baPAD was converting 4-coumaric acid into 4-hydroxystyrene. The codon usage was optimized for expression in E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Kang, S. Y., Choi, O., Lee, J. K., Ahn, J. O., Ahn, J. S., Hwang, B. Y., & Hong, Y. S. (2015). Artificial de novo biosynthesis of hydroxystyrene derivatives in a tyrosine overproducing Escherichia coli strain. Microbial cell factories, 14(1), 1-11. 10.1186/s12934-015-0268-7