Difference between revisions of "Part:BBa K3407013"
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<partinfo>BBa_K3407013 short</partinfo> | <partinfo>BBa_K3407013 short</partinfo> | ||
| − | T7 promoter lacking the last two GG. This is necessary to transcribe shRNA in vitro with RiboMAX express RNAi kit (Promega), as the template of the shRNA already has to be desinged by starting with GG. When coupled together, the transcript will be exactly what is designed as the shRNA. | + | T7 promoter lacking the last two GG. This is necessary to transcribe shRNA (<html><a href="https://parts.igem.org/Part:BBa_K3407006" target="_blank"><b>BBa_K3407006</b></a></html>) and shRNA* (<html><a href="https://parts.igem.org/Part:BBa_K3407007" target="_blank"><b>BBa_K3407007</b></a></html>) in vitro with RiboMAX express RNAi kit (Promega), as the template of the shRNA already has to be desinged by starting with GG. When coupled together, the transcript will be exactly what is designed as the shRNA. |
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Latest revision as of 14:36, 27 October 2020
T7 truncated promoter
T7 promoter lacking the last two GG. This is necessary to transcribe shRNA (BBa_K3407006) and shRNA* (BBa_K3407007) in vitro with RiboMAX express RNAi kit (Promega), as the template of the shRNA already has to be desinged by starting with GG. When coupled together, the transcript will be exactly what is designed as the shRNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]