Difference between revisions of "Part:BBa K3332062"
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<partinfo>BBa_K3332062 short</partinfo> | <partinfo>BBa_K3332062 short</partinfo> | ||
| − | This is an anchored proteins onto membranes through Lpp-OmpA and use GFP to comformation. We use | + | This is an anchored proteins onto membranes through Lpp-OmpA and use GFP to comformation. We use <partinfo>BBa_K880005<partinfo> and GFP to verify Lpp-OmpA's function which can anchor proteins onto membranes. |
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===Biology=== | ===Biology=== | ||
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Lpp-OmpA is an anchor protein from ''E. coli'', which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. | Lpp-OmpA is an anchor protein from ''E. coli'', which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. | ||
| − | EGFP is green fluorescent protein from jellyfish ''Aequorea Victoria'', which has been widely used as reporter for decades. EGFP is fused at N terminal with Lpp-OmpA so that EGFP may be displayed on the surface of E. coli.<ref>https://parts.igem.org/Part:BBa_E0420</ref><ref>http://2016.igem.org/Team:TJUSLS_China</ref> | + | EGFP is green fluorescent protein from jellyfish ''Aequorea Victoria'', which has been widely used as reporter for decades. EGFP is fused at N terminal with Lpp-OmpA so that EGFP may be displayed on the surface of ''E. coli''.<ref>https://parts.igem.org/Part:BBa_E0420</ref><ref>http://2016.igem.org/Team:TJUSLS_China</ref> |
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===Usage=== | ===Usage=== | ||
| − | Here, we used <partinfo>BBa_K880005</partinfo> to construct the expression system and obtained the composite part <partinfo>BBa_K3332062</partinfo>. Due to the limited time, we | + | |
| + | Here, we used <partinfo>BBa_K880005</partinfo> to construct the expression system and obtained the composite part <partinfo>BBa_K3332062</partinfo>. Due to the limited time, we did not get the gene in time. As a result, there is a lack of data about this part. The progress of this part remains in the stage of design. | ||
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<html> | <html> | ||
<figure> | <figure> | ||
| − | <img src="https://2020.igem.org/wiki/images/8/88/T--XMU-China--XMU-China_2020-J23100_B0034_Anchor_with_EGFP_fused_B0015.png" width=" | + | <img src="https://2020.igem.org/wiki/images/8/88/T--XMU-China--XMU-China_2020-J23100_B0034_Anchor_with_EGFP_fused_B0015.png" width="30%" style="float:center"> |
<figcaption> | <figcaption> | ||
<p style="font-size:1rem"> | <p style="font-size:1rem"> | ||
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</html> | </html> | ||
| − | Fig 1. Gene circuit of Lpp-OmpA-EGFP | + | :'''Fig 1.''' Gene circuit of Lpp-OmpA-EGFP. |
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===References=== | ===References=== | ||
<references/> | <references/> | ||
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Revision as of 14:34, 27 October 2020
J23100-RBS-Lpp-OmpA-GFP-terminator
This is an anchored proteins onto membranes through Lpp-OmpA and use GFP to comformation. We use No part name specified with partinfo tag. to construct the expression system and obtained the composite part BBa_K3332062. Due to the limited time, we did not get the gene in time. As a result, there is a lack of data about this part. The progress of this part remains in the stage of design.
- Fig 1. Gene circuit of Lpp-OmpA-EGFP.
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1170