Difference between revisions of "Part:BBa K3605001"
 
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Before we clone this gene,the software SnapGene viewer was used to analyze the sequence of GHK, its feature is showed as follows:
 
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Latest revision as of 14:01, 27 October 2020


Glucose hexokinase (GHK) from Gluconacetobacter xylinus

Glucose hexokinase (GHK) was originated from Gluconacetobacter xylinus CGMCC 2955. Using glucose, this enzyme catalyzes the formation of glucose-6-phosphate, which is the glycolytic intermediate and the origin of substrate synthesis for Bacterial cellulose production.

K3605001-1.jpg



Glucose hexokinase (GHK) was amplified using PCR method, and inserted into the vector pSB1C3. The result was identified in figure 1.

K3605001-3.jpg

Fig.1. The result of Glucose hexokinase (GHK) gene cloning. M: Marker; 1: PCR result of GHK; 2: Digestion of pSB1C3 containing GHK.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 548
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 478