Difference between revisions of "Part:BBa K3544003:Design"

(Design Notes)
(Source)
Line 11: Line 11:
 
===Source===
 
===Source===
  
Botrytis Cinerea
+
The original nucleotide sequence is from NCBI <i>Botrytis cinerea B05.10 chromosome 8, NC_037317.1</i>. The sequence is optimized according to the condon usage bias in <i>Saccharomyces cerevisiae</i> and synthesized from Sangon.
  
 
===References===
 
===References===

Revision as of 13:59, 27 October 2020


Coding sequence of BcABA4, a member in biosynthetic gene cluster of ABA in B.cinerea


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 696
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 696
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 696
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 696
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze α-ionylideneacetic acid into abscisic acid.

Source

The original nucleotide sequence is from NCBI Botrytis cinerea B05.10 chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in Saccharomyces cerevisiae and synthesized from Sangon.

References