Difference between revisions of "Part:BBa K3468072"
| Line 12: | Line 12: | ||
<partinfo>BBa_K3468072 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3468072 SequenceAndFeatures</partinfo> | ||
Adding hydrogen bonds to stabilize α5-β7loop | Adding hydrogen bonds to stabilize α5-β7loop | ||
| + | |||
| + | The reduction of DDG was predicted by FoldX. PyOML was used for visual screening. it formed hydrogen bonds with 186-HIS and 190-ASN. after mutation to ASN, it formed hydrogen bonds with 190-ASN, which stabilized α5-β7loop and was beneficial to improve the thermal stability of PETase. | ||
| + | [[File:S188N.png|400px|thumb|left|Fig.1 S188N in PyMOL]] | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 13:59, 27 October 2020
PETase S188N
The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from S to N at 188 position which can be more stable in higher temperature compared with the wild type.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Adding hydrogen bonds to stabilize α5-β7loop
The reduction of DDG was predicted by FoldX. PyOML was used for visual screening. it formed hydrogen bonds with 186-HIS and 190-ASN. after mutation to ASN, it formed hydrogen bonds with 190-ASN, which stabilized α5-β7loop and was beneficial to improve the thermal stability of PETase.
