Difference between revisions of "Part:BBa K3402061:Design"
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===Design Notes=== | ===Design Notes=== | ||
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Latest revision as of 13:56, 27 October 2020
The Cas9 and sgRNA expression cassette
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 5603
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1730
Illegal BglII site found at 5294
Illegal XhoI site found at 1051
Illegal XhoI site found at 1780
Illegal XhoI site found at 2143
Illegal XhoI site found at 5095
Illegal XhoI site found at 6695 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 5603
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 5603
Illegal NgoMIV site found at 2557
Illegal NgoMIV site found at 3661 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1501
Illegal BsaI site found at 2218
Design Notes
Source
Starmerella bombicola