Difference between revisions of "Part:BBa K3332013"
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We anchor GRHPR protein onto membranes through Lpp-OmpA to catalyze the reaction of reducing glyoxalic acid and consuming NADPH. We use K880005 to construct the expression system and anchor GRHPR on the surface of E.coli. | We anchor GRHPR protein onto membranes through Lpp-OmpA to catalyze the reaction of reducing glyoxalic acid and consuming NADPH. We use K880005 to construct the expression system and anchor GRHPR on the surface of E.coli. | ||
| − | < | + | ===Biology=== |
| − | === | + | |
| + | Lpp-OmpA is an anchor protein from E. coli, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. GRHPR, a Glyoxylate reductase from human liver, can reduce glyoxylic acid when NADPH is used as cofactor. GRHPR is fused at N terminal with Lpp-OmpA so that GRHPR can be displayed on the surface of E. coli.<ref>Rumsby G, Cregeen D P. Identification and expression of a cDNA for human hydroxypyruvate/glyoxylate reductase[J]. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1999, 1446(3): 383-388.</ref><ref>http://2016.igem.org/Team:TJUSLS_China</ref> | ||
| + | <html> | ||
| + | <figure> | ||
| + | <img src="https://2020.igem.org/wiki/images/8/82/T--XMU-China--XMU-China_2020-GRHPR%E9%94%9A%E5%AE%9A.png" width="45%" style="float:center"> | ||
| + | <figcaption> | ||
| + | <p style="font-size:1rem"> | ||
| + | </p> | ||
| + | </figcaption> | ||
| + | </figure> | ||
| + | </html> | ||
| + | |||
| + | Fig 1. mechanism | ||
| + | |||
| + | ===Usage=== | ||
| + | |||
| + | Here, we used K880005 to construct the expression system and demonstrate the effect of Lpp-OmpA-GRHPR on the surface of E. coli. We obtained the composite part BBa_K3332058. Due to the limited time, we didn’t get the gene in time. As a result, there is a lack of data about this part. The progress of this part remains in the stage of design. | ||
| + | <html> | ||
| + | <figure> | ||
| + | <img src="https://2020.igem.org/wiki/images/a/aa/T--XMU-China--XMU-China_2020-J23100_B0034_lpp-ompA-grhpr_B0015.png" width="45%" style="float:center"> | ||
| + | <figcaption> | ||
| + | <p style="font-size:1rem"> | ||
| + | </p> | ||
| + | </figcaption> | ||
| + | </figure> | ||
| + | </html> | ||
| + | |||
| + | Fig 2. Gene circuit of Lpp-OmpA-GRHPR | ||
| + | ===References=== | ||
| + | <references/> | ||
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Revision as of 13:55, 27 October 2020
Lpp-OmpA-GRHPR
We anchor GRHPR protein onto membranes through Lpp-OmpA to catalyze the reaction of reducing glyoxalic acid and consuming NADPH. We use K880005 to construct the expression system and anchor GRHPR on the surface of E.coli.
Biology
Lpp-OmpA is an anchor protein from E. coli, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. GRHPR, a Glyoxylate reductase from human liver, can reduce glyoxylic acid when NADPH is used as cofactor. GRHPR is fused at N terminal with Lpp-OmpA so that GRHPR can be displayed on the surface of E. coli.[1][2]
Fig 1. mechanism
Usage
Here, we used K880005 to construct the expression system and demonstrate the effect of Lpp-OmpA-GRHPR on the surface of E. coli. We obtained the composite part BBa_K3332058. Due to the limited time, we didn’t get the gene in time. As a result, there is a lack of data about this part. The progress of this part remains in the stage of design.
Fig 2. Gene circuit of Lpp-OmpA-GRHPR
References
- ↑ Rumsby G, Cregeen D P. Identification and expression of a cDNA for human hydroxypyruvate/glyoxylate reductase[J]. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1999, 1446(3): 383-388.
- ↑ http://2016.igem.org/Team:TJUSLS_China
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1012
- 1000COMPATIBLE WITH RFC[1000]