Difference between revisions of "Part:BBa K143012"

(→‎Promoter veg: Constitutive Promoter for B. subtilis)
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<span class='h3bb'><big>'''Part Characterisation'''</big></span>
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Please see the testing constructs [https://parts.igem.org/Part:BBa_K143079 BBa_K143079] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K143082 BBa_K143082] for characterisation data.
  
 
===References===
 
===References===

Revision as of 00:44, 30 October 2008

Promoter veg: Constitutive Promoter for B. subtilis


Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. Pveg contains binding sites for the B. sutbilis major sigma factor, Sigma factor A 1. Pveg in B. subtilis utilises two binding sites to cause high expression of genes #2, however this Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulation factor spoOA interacts with Pveg though it is not known how #3. The context with which we used the promoter Pveg is as a Polymerase Per Second (PoPS) generator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




Part Characterisation

Please see the testing constructs BBa_K143079 and BBa_K143082 for characterisation data.

References

<biblio>

  1. 1 pmid=6181373
  2. 2 pmid=6820069
  3. 3 pmid=14651647

</biblio>