Difference between revisions of "Part:BBa K3402061"

Revision as of 13:55, 27 October 2020

The Cas9 and sgRNA expression cassette

This device is composed of Ptef1 (BBa_K3402007), Cas9 (BBa_K3402023), Tsyn7 (BBa_K3402001) and sgPXA1 (BBa_K3402008).

Usage and Biology

The use of the strongest promoter Ptef1 to express Cas9 protein and sgRNA could increase the gene-editing efficiency. sgPXA1 will guide the Cas9 to edit the PXA1 site.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 5603
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1730
Illegal BglII site found at 5294
Illegal XhoI site found at 1051
Illegal XhoI site found at 1780
Illegal XhoI site found at 2143
Illegal XhoI site found at 5095
Illegal XhoI site found at 6695
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 5603
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 5603
Illegal NgoMIV site found at 2557
Illegal NgoMIV site found at 3661
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1501
Illegal BsaI site found at 2218

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3402061 short</partinfo>
-This device is composed of Ptef1 (BBa_K3402007), Cas9 (BBa_K3402023), Tsyn7 (BBa_K3402001) and sgPXA1 (BBa_K3402008).
+This device is composed of P<i>tef1</i> ([https://parts.igem.org/Part:BBa_K3402007# BBa_K3402007]), <i>Cas9</i> ([https://parts.igem.org/Part:BBa_K3402023# BBa_K3402023]), T<i>syn7</i> ([https://parts.igem.org/Part:BBa_K3402001# BBa_K3402001]) and sg<i>PXA1</i> ([https://parts.igem.org/Part:BBa_K3402008# BBa_K3402008]).
+[[Image:The Cas9 and sgRNA expression cassette.png|400px|]]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The use of the strongest promoter P<i>tef1</i> to express <i>Cas9</i> protein and sgRNA could increase the gene-editing efficiency. sg<i>PXA1</i> will guide the Cas9 to edit the PXA1 site.
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