Difference between revisions of "Part:BBa K3365011"

 
 
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<partinfo>BBa_K3365011 short</partinfo>
 
<partinfo>BBa_K3365011 short</partinfo>
  
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the transcription of sgRNA
+
The target and sgRNA scaffold sequence are located directly downstream of the promoter J23119(BBa_J23119). This part is used as an sgRNA production module.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:51, 27 October 2020


sgRNA production module

The target and sgRNA scaffold sequence are located directly downstream of the promoter J23119(BBa_J23119). This part is used as an sgRNA production module.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]