Difference between revisions of "Part:BBa K3384313"

Revision as of 13:46, 27 October 2020

pprm1 Pro

Multiple copies of putative Ste12 binding sites are prevalent in pheromone-responsive promoters of Saccharomyces cerevisiae, which are also known as pheromone response elements (PREs). pprm1 contains 3 × PREs which is in the opposite direction of the promoter. pprm1 Pro is a modified pheromone-responsive promoter. It contains 3 × PREs in the same direction of the promoter.

Characterization

When assembled with promotor BBa_K3384314, BBa_K3112009, and BBa_K3384311 in pRS415, this construct expressed GFP. Then the activity of these promoters can be quantitatively measured through flow cytometer. As is shown in figure 1, the pheromone concentration has no significant effect on the GFP expression intensity under the control of pprm1 Pro, indicating that this engineered pprm1 remains a stable expression level when induced by pheromone. With a low and stable induced expression level, this part assembled with reporter genes, for example, chromoproteins, can be used in co-expression scenarios with some colored products, and its weak expression intensity will not affect the observation of its colored products.

Reference

1. LSengupta, P., and Cochran, B. H. (1990) The Pre and Pq Box Are Functionally Distinct Yeast Pheromone Response Elements, Molecular and Cellular Biology 10, 6809-6812.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3384313 short</partinfo>
-Multiple copies of putative Ste12 binding sites are prevalent in pheromone-responsive promoters of Saccharomyces cerevisiae, which are also known as pheromone response elements (PREs). pprm1 contains 3 × PREs which is in the opposite direction of the promoter. p<em>prm1</em> Pro is a modified pheromone-responsive promoter. It  contains 3 × PREs in the same direction of the promoter.
+Multiple copies of putative Ste12 binding sites are prevalent in pheromone-responsive promoters of Saccharomyces cerevisiae, which are also known as pheromone response elements (PREs). p<em>prm1</em> contains 3 × PREs which is in the opposite direction of the promoter. p<em>prm1</em> Pro is a modified pheromone-responsive promoter. It  contains 3 × PREs in the same direction of the promoter.
 ===Characterization===
-When assembled with promotor <partinfo>BBa_K3384314</partinfo>, <partinfo>BBa_K3112009</partinfo>, and <partinfo>BBa_K3384311</partinfo> in pRS415, this construct expressed GFP. Then the activity of these promoters can be quantitatively measured through flow cytometer. As is shown in figure 1, the pheromone concentration has no significant effect on the GFP expression intensity under the control of pprm1 Pro, indicating that this engineered pprm1 remains a stable expression level when induced by pheromone. With a low and stable induced expression level, this part assembled with reporter genes, for example, chromoproteins, can be used in co-expression scenarios with some colored products, and its weak expression intensity will not affect the observation of its colored products.
+When assembled with promotor <partinfo>BBa_K3384314</partinfo>, <partinfo>BBa_K3112009</partinfo>, and <partinfo>BBa_K3384311</partinfo> in pRS415, this construct expressed GFP. Then the activity of these promoters can be quantitatively measured through flow cytometer. As is shown in figure 1, the pheromone concentration has no significant effect on the GFP expression intensity under the control of p<em>prm1</em> Pro, indicating that this engineered p<em>prm1</em> remains a stable expression level when induced by pheromone. With a low and stable induced expression level, this part assembled with reporter genes, for example, chromoproteins, can be used in co-expression scenarios with some colored products, and its weak expression intensity will not affect the observation of its colored products.