Difference between revisions of "Part:BBa K3544005:Design"

Revision as of 13:31, 27 October 2020

yeGFP: a green fluorescent protein in S. cerevisiae

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

2020 SCU-China use this protein as a reporter in the yeast. The fluorescence of yeGFP can be detected easily and can be distinguished with DsRed. For more information, please view https://2020.igem.org/Team:SCU-China/Design.

Source

Artificial modification from GFP

References

Chen J, Zhou J, Bae W, Sanders CK, Nolan JP, Cai H. A yEGFP-based reporter system for high-throughput yeast two-hybrid assay by flow cytometry. Cytometry A. 2008 Apr;73(4):312-20. doi: 10.1002/cyto.a.20525. PMID: 18307271.

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	===References===		===References===
		+	Chen J, Zhou J, Bae W, Sanders CK, Nolan JP, Cai H. A yEGFP-based reporter system for high-throughput yeast two-hybrid assay by flow cytometry. Cytometry A. 2008 Apr;73(4):312-20. doi: 10.1002/cyto.a.20525. PMID: 18307271.