Difference between revisions of "Part:BBa K3365053"

Latest revision as of 12:56, 27 October 2020

RFP with double terminator

This part is used as a reporter for detecting whether dCas9-ωis on-target. We integrated this part downstream of transcription activating unit. If dCas9-ω is binding to target sequence, this part will be activated to express and the cell then emit red fluorescence, allowing us to know that dCas9-ωis literally on-target. As we plan to put the potential off-target site downstream of this part, we added a terminator to RFP so that the expression of this part can stop before next off-target reporting site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 700
Illegal XhoI site found at 691
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 555
Illegal AgeI site found at 667
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3365053 short</partinfo>
-This part is used as a reporter for detecting whether dCas9-ωis on-target. We integrated this part downstream of transcription activating unit. If dCas9-ω is binding to target sequence, this part will be activated to express and the cell then emit red fluorescence, allowing us to know that dCas9-ωis on-target. As we plan to put the potential off-target site downstream of this part, we added a terminator to RFP so that the expression of this part can stop before next off-target reporting site.
+This part is used as a reporter for detecting whether dCas9-ωis on-target. We integrated this part downstream of transcription activating unit. If dCas9-ω is binding to target sequence, this part will be activated to express and the cell then emit red fluorescence, allowing us to know that dCas9-ωis literally on-target. As we plan to put the potential off-target site downstream of this part, we added a terminator to RFP so that the expression of this part can stop before next off-target reporting site.
 <!-- Add more about the biology of this part here