Difference between revisions of "Part:BBa K3365052"
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The RFP is one reporter of our system. Its excitation wavelength is 555nm and emission wavelength is 583nm in measuring its expression level by microplate spectrophotometer. | The RFP is one reporter of our system. Its excitation wavelength is 555nm and emission wavelength is 583nm in measuring its expression level by microplate spectrophotometer. | ||
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| + | We put this part downstream of transcription unit as reporter, as we can easily know the expression level of it by detecting fluorescence intensity. dCas9 protein with subnit Omega binding to target site and subnit Omega would promote the expression of this part. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Latest revision as of 12:53, 27 October 2020
RFP
The RFP is one reporter of our system. Its excitation wavelength is 555nm and emission wavelength is 583nm in measuring its expression level by microplate spectrophotometer.
We put this part downstream of transcription unit as reporter, as we can easily know the expression level of it by detecting fluorescence intensity. dCas9 protein with subnit Omega binding to target site and subnit Omega would promote the expression of this part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]