Difference between revisions of "Part:BBa K3468027"
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===Functional Parameters=== | ===Functional Parameters=== | ||
| + | The benzene ring is located in a gully between two aromatic residues TYR87 and TRP185. | ||
| + | The distance of PI-PI bond is 4.7A between TRP185 and the benzene ring in part of the first MHET. | ||
| + | The whole ligand just fits into the pocket and binds tightly. | ||
| + | Three residues of Ser160, Asp206 and His237 formed a catalytic triplet, and ranging was performed: Y87:4.7A ;M161:5.1A; H237:4.7A. | ||
| + | |||
| + | Significance of molecular docking for mutant work: | ||
| + | The molecular docking model can predict the binding mode and affinity of mutants and ligands, and promote the work of mutants by analyzing the result model. | ||
| + | |||
<partinfo>BBa_K3468027 parameters</partinfo> | <partinfo>BBa_K3468027 parameters</partinfo> | ||
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Revision as of 12:32, 27 October 2020
PETase A40P,S54W,A80V,T113P,T116R,Q119D,Q127L,S193R,S214F,K252L,T266P,N275F
The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from A40P,S54W,A80V,T113P,T116R,Q119D,Q127L,S193R,S214F,K252L,T266P,N275F position which can be more stable in higher temperature compared with the wild type.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 240
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 240
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 240
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 240
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 240
- 1000COMPATIBLE WITH RFC[1000]