Difference between revisions of "Part:BBa K3484001"

Revision as of 11:58, 27 October 2020

Intein-mediated T3(THRB) → sfGFP (transcriptional unit)

This part is the transcriptional unit for BBa_K3484000. This part expresses constitutively an intein-mediated T3 biosensor. It is conformed by the Mycobacterium tuberculosis (Mtu) RecA intein, but replacing its endonuclease domain by the thyroid hormone receptor β (THR-β) ligand binding domain (LBD). The extein (the side proteins that the intein will splice and join) is conformed by super-folder GFP (sfGFP). In presence of T3, the intein domains splice the protein and full sfGFP is generated.

To see the full information and characterization, take a loop at the protein domain BBa_K3484000.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 235
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 722
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 278
Illegal SapI.rc site found at 1637

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3484001 short</partinfo>
-This part is the transcriptional unit for [https://parts.igem.org/Part:BBa_K3484000 BBa_K3484000]. This part expresses constitutively an intein-mediated T3 biosensor. It is conformed by the Mycobacterium tuberculosis (Mtu) RecA intein, but replacing its endonuclease domain by the thyroid hormone receptor &#946; (THR-&#946;) ligand binding domain (LBD). The extein (the side proteins that the intein will splice and join) is conformed by super-folder GFP (sfGFP). In presence of T3, the intein domains splice the protein and full sfGFP is generated.
+This part is the transcriptional unit for <partinfo>BBa_K3484000</partinfo>. This part expresses constitutively an intein-mediated T3 biosensor. It is conformed by the Mycobacterium tuberculosis (Mtu) RecA intein, but replacing its endonuclease domain by the thyroid hormone receptor &#946; (THR-&#946;) ligand binding domain (LBD). The extein (the side proteins that the intein will splice and join) is conformed by super-folder GFP (sfGFP). In presence of T3, the intein domains splice the protein and full sfGFP is generated.
 To see the full information and characterization, take a loop at the protein domain <partinfo>BBa_K3484000</partinfo>.