Difference between revisions of "Part:BBa K3506099"
Line 4: Line 4:
  
 
Generally, <i>GAPDH</i> promoter can drive the expression of glyceraldehyde-3-phosphate dehydrogenase gene in many species.
 
Generally, <i>GAPDH</i> promoter can drive the expression of glyceraldehyde-3-phosphate dehydrogenase gene in many species.
 +
  
 
<b><font size"3">Biology and Usage</font></b>
 
<b><font size"3">Biology and Usage</font></b>
  
−
<i>GAPDH</i> mRNA accounts for 2–5% of the poly(A) RNA in yeasts, suggesting that GAPDH promoter is a highly active promoter. In our project, <i>GAPDH</i> promoter is used to effectively transcribe the genes that contains <i>U6</i> promoter and gRNA. General vectors have been designed that rely on homologous <i>GAPDH</i> promoter regions to drive the expressions of heterologous genes in yeasts and other filamentous fungi.
+
<i>GAPDH</i> mRNA accounts for 2–5% of the poly(A) RNA in yeasts, suggesting that GAPDH promoter is a highly active promoter. In our project, <i>GAPDH</i> promoter is used to effectively transcribe the genes that contains <i>U6</i> promoter and hgRNA. General vectors have been designed that rely on homologous <i>GAPDH</i> promoter regions to drive the expressions of heterologous genes in yeasts and other filamentous fungi.
  
 
<!-- -->
 
<!-- -->
Line 16: Line 17:
 
<b><font size"3">Reference</font></b>
 
<b><font size"3">Reference</font></b>
  
−
[1]Jia Y, Li S, Allen G, Feng S, Xue L. A novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter for expressing transgenes in the halotolerant alga Dunaliella salina. Curr Microbiol. 2012 May;64(5):506-13. doi: 10.1007/s00284-012-0102-y. Epub 2012 Feb 28. PMID: 22371187.
+
[1]Jia Y, Li S, Allen G, Feng S, Xue L. A novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter for expressing transgenes in the halotolerant alga Dunaliella salina. Curr Microbiol. 2012 May;64(5):506-13.
  
  

Revision as of 11:57, 27 October 2020


GAPDH promoter

Generally, GAPDH promoter can drive the expression of glyceraldehyde-3-phosphate dehydrogenase gene in many species.


Biology and Usage

GAPDH mRNA accounts for 2–5% of the poly(A) RNA in yeasts, suggesting that GAPDH promoter is a highly active promoter. In our project, GAPDH promoter is used to effectively transcribe the genes that contains U6 promoter and hgRNA. General vectors have been designed that rely on homologous GAPDH promoter regions to drive the expressions of heterologous genes in yeasts and other filamentous fungi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 436


Reference

[1]Jia Y, Li S, Allen G, Feng S, Xue L. A novel glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter for expressing transgenes in the halotolerant alga Dunaliella salina. Curr Microbiol. 2012 May;64(5):506-13.