Difference between revisions of "Part:BBa K3360004:Design"

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===Design Notes===
 
===Design Notes===
−
Encoding the endo-beta-1,4-glucanase of Streptomyces,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
+
Encoding the endo-beta-1,4-glucanase CL34 of Streptomyces,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
  
 
===Source===
 
===Source===
−
Encoding the endo-beta-1,4-glucanase of Streptomyces,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
+
Encoding the endo-beta-1,4-glucanase of CL34 Streptomyces,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.
  
 
===References===
 
===References===

Latest revision as of 11:57, 27 October 2020


endo-beta-1_4-glucanase from Streptomyces


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 431
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Encoding the endo-beta-1,4-glucanase CL34 of Streptomyces,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.

Source

Encoding the endo-beta-1,4-glucanase of CL34 Streptomyces,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.

References