Difference between revisions of "Part:BBa K2904111"

Revision as of 11:23, 27 October 2020

aTc inducible Tet promoter

This promoter is induced by aTc. It was used by 2019 OUC-China team.

OUC-China 2020’s characterization

Design

It is very important for us to select the inducible promoter with low transcriptional leakage and high dynamic range. These promoters are essential parts of building the logic gates. These promoters also played a crucial role in the subsequent construction of multiple-input logic gates and swapping gates.

Result

The Tet promoter fluorescence data in E. coli BL21 (DE3) at different aTc concentrations (0mg/mL, 0.01mg/mL, 0.1mg/mL, 0.25mg/mL, 0.5mg/mL and 1mg/mL). The Tet promoter showed the highest fluorescence intensity when the aTc concentration was 0.25mg/mL.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 13: / Line 13: @@
 https://2020.igem.org/wiki/images/thumb/8/86/T--OUC-China--result_fig1A_pTet.jpg/800px-T--OUC-China--result_fig1A_pTet.jpg
-The Tet promoter fluorescence data in BL21 (DE3) at different aTc concentrations (0mg/ml, 0.01mg/ml, 0.1mg/ml, 0.25mg/ml, 0.5mg/ml and 1mg/ml). The Tet promoter showed the highest fluorescence intensity when the aTc concentration was 0.25mg/ml.
+The Tet promoter fluorescence data in E. coli BL21 (DE3) at different aTc concentrations (0mg/mL, 0.01mg/mL, 0.1mg/mL, 0.25mg/mL, 0.5mg/mL and 1mg/mL). The Tet promoter showed the highest fluorescence intensity when the aTc concentration was 0.25mg/mL.
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