Difference between revisions of "Part:BBa K3407018:Design"

 
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<partinfo>BBa_K3407018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3407018 SequenceAndFeatures</partinfo>
  
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===Design notes===
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The biobrick is made with EcoRI and BglII before the RBS and CDS, and BamHI and XhoI after the CDS, so the piece CDS can be excised using these set of enzymes.
  
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===Design Notes===
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===Source===
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Hello
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Genomic DNA from <i>B. subtilis</i> strain 168.
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Plasmid used pBbE8c backbone from BglBricks. pBbE8c-RFP was a gift from Jay Keasling (Addgene plasmid # 35269; http://n2t.net/addgene:35269; RRID:Addgene_35269) <html><a href="#1">[1]</a></html>
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Please refer to the Addgene page for more information about licences associated with the use of the plasmid.
  
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===References===
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<html>
  
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===Source===
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<head>
  
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Hello
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<title>Ordered List</title>
  
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===References===
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<a id="1" href="https://jbioleng.biomedcentral.com/articles/10.1186/1754-1611-5-12" target="_blank">
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BglBrick vectors and datasheets: A synthetic biology platform for gene expression. Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, Keasling JD. J Biol Eng. 2011 Sep 20;5:12. 10.1186/1754-1611-5-12 PubMed 21933410</a>
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</html>

Revision as of 11:19, 27 October 2020


Mini-3 endoribonuclease with araBAD promoter - RBS - and rrnBT1 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 300
    Illegal XbaI site found at 673
    Illegal PstI site found at 527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 300
    Illegal PstI site found at 527
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 300
    Illegal BglII site found at 309
    Illegal BamHI site found at 809
    Illegal XhoI site found at 818
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 300
    Illegal XbaI site found at 673
    Illegal PstI site found at 527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 300
    Illegal XbaI site found at 673
    Illegal PstI site found at 527
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56

Design notes

The biobrick is made with EcoRI and BglII before the RBS and CDS, and BamHI and XhoI after the CDS, so the piece CDS can be excised using these set of enzymes.

Source

Genomic DNA from B. subtilis strain 168. Plasmid used pBbE8c backbone from BglBricks. pBbE8c-RFP was a gift from Jay Keasling (Addgene plasmid # 35269; http://n2t.net/addgene:35269; RRID:Addgene_35269) [1] Please refer to the Addgene page for more information about licences associated with the use of the plasmid.

References

Ordered List

  1. BglBrick vectors and datasheets: A synthetic biology platform for gene expression. Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, Keasling JD. J Biol Eng. 2011 Sep 20;5:12. 10.1186/1754-1611-5-12 PubMed 21933410