Difference between revisions of "Part:BBa K3331004"
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pgmA [Pseudomonas aeruginosa PAO1 (strain: PAO1)] | pgmA [Pseudomonas aeruginosa PAO1 (strain: PAO1)] | ||
<div>Encodes Phosphomannomutase, a highly reversible phosphoryltransferase that catalyzes the interconversion of α-D-mannose 1-phosphate and D-mannose 6-phosphate.</div> | <div>Encodes Phosphomannomutase, a highly reversible phosphoryltransferase that catalyzes the interconversion of α-D-mannose 1-phosphate and D-mannose 6-phosphate.</div> | ||
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This part comes from the genome of Pseudomonas aeruginosa. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis. | This part comes from the genome of Pseudomonas aeruginosa. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis. | ||
Latest revision as of 10:56, 27 October 2020
P.A. pgmA
pgmA [Pseudomonas aeruginosa PAO1 (strain: PAO1)]
Encodes Phosphomannomutase, a highly reversible phosphoryltransferase that catalyzes the interconversion of α-D-mannose 1-phosphate and D-mannose 6-phosphate.
This part comes from the genome of Pseudomonas aeruginosa. We optimized the sequence with the codon preferred by Bacillus subtilis. In this way, it can be more efficiently expressed in Bacillus subtilis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 672
Illegal BglII site found at 705 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 27
Illegal AgeI site found at 261
Illegal AgeI site found at 900
Illegal AgeI site found at 1278 - 1000COMPATIBLE WITH RFC[1000]
Characterization
We cloned and confirmed the target fragment by PCR. The length of our fragment is 1392bp. The following results showed the successful result.
