Difference between revisions of "Part:BBa K3331003"

(Characterization)
Line 3: Line 3:
 
<partinfo>BBa_K3331003 short</partinfo>
 
<partinfo>BBa_K3331003 short</partinfo>
  
This part catalyzes the interconversion of alpha-D-glucose 1-phosphate and D-glucose 6-phosphate.
+
3.pgmA [Bacillus subtilis (strain 168)]
This part comes from the genome of  Bacillus subtilis.
+
<div>Encodes 2,3-bisphosphoglycerate-independent phosphoglycerate mutase. This enzyme catalyzes the reversible reaction between 2-phosphoglycerate and 3-phosphoglycerate</div>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:52, 27 October 2020


B.S. pgmA

3.pgmA [Bacillus subtilis (strain 168)]

Encodes 2,3-bisphosphoglycerate-independent phosphoglycerate mutase. This enzyme catalyzes the reversible reaction between 2-phosphoglycerate and 3-phosphoglycerate

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

We cloned and confirmed the target fragment by PCR. The length of our fragment is 1875bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.